sinv 6k Search Results


sinv 6k  (Addgene inc)
90
Addgene inc sinv 6k
Generation and growth kinetics of <t>SINV</t> <t>6K</t> ion-channel chimeras. ( A ) The amino acid sequences of 6K and 6K mutant chimeras—WT SINV, ∆6K SINV, SINV-ETM, SINV-ETM (N15A), SINV-ETM (V25F), and SINV-ETM (N15A, V25F). The ETM sequence is shown in red with channel-inactivating mutations in blue. ( B ) Plaque morphologies of viruses mentioned in ( A ) grown in BHK-21 cells for 2 days after electroporation. ( C ) Differences in mean plaque diameters of 10 randomly selected plaques for every mutant virus were compared to WT SINV using GraphPad Prism software. Dunnett’s multiple comparisons test as part of one-way ANOVA was used to determine statistical significance with a 95% confidence interval. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 and ns-not significant. ( D ) One-step growth curves of wild-type and channel mutant viruses over 12 hours of infection in BHK-21 cells. Cells were infected at an MOI of 1, and virus-containing media were harvested at given time points to indicate the rate of virus release per hour. Data are representative of two independent experiments. ( E ) Comparison of viral titers and the total number of viral RNA genomes produced by wild-type and mutant viruses 12 hours after electroporation with in vitro transcribed RNA. The experiment was performed in triplicate, and error bars represent the standard deviation (SD). ( F ) One-step growth curves of wild-type and mCherry-tagged SINV and SINV-ETM chimera at an MOI of 2. Data are representative of two independent experiments.
Sinv 6k, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sinv 6k/product/Addgene inc
Average 90 stars, based on 1 article reviews
sinv 6k - by Bioz Stars, 2026-03
90/100 stars
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ip6k1 antibody rabbit pab antigen affinity purified  (Sino Biological)
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Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human IP6K1 and purified by antigen affinity chromatography
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human rsk4 rps6ka6 protein  (Sino Biological)
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A DNA sequence encoding the full length of human RSK4 NP 055311 1 Met 1 Leu 745 was fused with the GST tag at the N terminus
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ps6k rps6kb1 antibody rabbit pab antigen affinity purified  (Sino Biological)
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Produced in rabbits immunized with purified recombinant Human PS6K RPS6KB1 rh PS6K RPS6KB1 Catalog 10099 H09B P23443 Met1 Leu525 PS6K RPS6KB1 specific IgG was purified by Human PS6K RPS6KB1 affinity chromatography
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sars cov 2 spike s1 t95i y144s y145n r346k e484k n501y d614g p681h protein  (Sino Biological)
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A DNA sequence encoding the SARS CoV 2 Spike S1 YP 009724390 1 with mutations T95I Y144S Y145N R346K E484K N501Y D614G P681H Met1 Arg685 was expressed with a polyhistidine tag at the C terminus
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human rps6ka1 gene orf cdna clone expression plasmid  (Sino Biological)
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Full length Clone DNA of Human ribosomal protein S6 kinase A1
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human ps6k rps6kb1 gene orf cdna clone expression plasmid c gfpspark tag  (Sino Biological)
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Full length Clone DNA of Human ribosomal protein S6 kinase 70kDa polypeptide 1 with C terminal GFPSpark tag
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recombinant human hydroxylysine kinase his tagged  (Creative BioMart)
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HYKK 1 373aa Human His tag E coliMGSSHHHHHH SSGLVPRGSH MGSMSSGNYQ QSEALSKPTF SEEQASALVE SVFGLKVSKV RPLPSYDDQN FHVYVSKTKD GPTEYVLKIS NTKASKNPDL IEVQNHIIMF LKAAGFPTAS VCHTKGDNTA SLVSVDSGSE IKSYLVRLLT YLPGRPIAEL PVSPQLLYEI GKLAAKLDKT LQRFHHPKLS SLHRENFIWN LKNVPLLEKY LYALGQNRNR EIVEHVIHLF KEEVMTKLSH FRECINHGDL NDHNILIESS KSASGNAEYQ VSGILDFGDM
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xpress human ip6k1 over expressing stable cell line  (AcceGen Biotechnology)
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AcceGen can provide custom lentiviral constructs expressing any genes of interest as long as it is less than about 3 kb Lentiviral technology enables us to efficiently generate stable expression lines which are then selected
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s6k1 rps6kb1 antibody rabbit pab antigen affinity purified  (Sino Biological)
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Produced in rabbits immunized with a synthetic peptide corresponding to the N terminus of the Human S6K1 RPS6KB1 and purified by antigen affinity chromatography
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human ps6k rps6kb1 gene orf cdna clone in cloning vector  (Sino Biological)
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Full length Clone DNA of Human ribosomal protein S6 kinase 70kDa polypeptide 1
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human rsk3 rps6ka2 gene orf cdna clone expression plasmid c myc tag  (Sino Biological)
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Full length Clone DNA of Human ribosomal protein S6 kinase 90kDa polypeptide 2 with C terminal Myc tag
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Image Search Results


Generation and growth kinetics of SINV 6K ion-channel chimeras. ( A ) The amino acid sequences of 6K and 6K mutant chimeras—WT SINV, ∆6K SINV, SINV-ETM, SINV-ETM (N15A), SINV-ETM (V25F), and SINV-ETM (N15A, V25F). The ETM sequence is shown in red with channel-inactivating mutations in blue. ( B ) Plaque morphologies of viruses mentioned in ( A ) grown in BHK-21 cells for 2 days after electroporation. ( C ) Differences in mean plaque diameters of 10 randomly selected plaques for every mutant virus were compared to WT SINV using GraphPad Prism software. Dunnett’s multiple comparisons test as part of one-way ANOVA was used to determine statistical significance with a 95% confidence interval. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 and ns-not significant. ( D ) One-step growth curves of wild-type and channel mutant viruses over 12 hours of infection in BHK-21 cells. Cells were infected at an MOI of 1, and virus-containing media were harvested at given time points to indicate the rate of virus release per hour. Data are representative of two independent experiments. ( E ) Comparison of viral titers and the total number of viral RNA genomes produced by wild-type and mutant viruses 12 hours after electroporation with in vitro transcribed RNA. The experiment was performed in triplicate, and error bars represent the standard deviation (SD). ( F ) One-step growth curves of wild-type and mCherry-tagged SINV and SINV-ETM chimera at an MOI of 2. Data are representative of two independent experiments.

Journal: Journal of Virology

Article Title: A BSL-2 chimeric system designed to screen SARS-CoV-2 E protein ion channel inhibitors

doi: 10.1128/jvi.02252-24

Figure Lengend Snippet: Generation and growth kinetics of SINV 6K ion-channel chimeras. ( A ) The amino acid sequences of 6K and 6K mutant chimeras—WT SINV, ∆6K SINV, SINV-ETM, SINV-ETM (N15A), SINV-ETM (V25F), and SINV-ETM (N15A, V25F). The ETM sequence is shown in red with channel-inactivating mutations in blue. ( B ) Plaque morphologies of viruses mentioned in ( A ) grown in BHK-21 cells for 2 days after electroporation. ( C ) Differences in mean plaque diameters of 10 randomly selected plaques for every mutant virus were compared to WT SINV using GraphPad Prism software. Dunnett’s multiple comparisons test as part of one-way ANOVA was used to determine statistical significance with a 95% confidence interval. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 and ns-not significant. ( D ) One-step growth curves of wild-type and channel mutant viruses over 12 hours of infection in BHK-21 cells. Cells were infected at an MOI of 1, and virus-containing media were harvested at given time points to indicate the rate of virus release per hour. Data are representative of two independent experiments. ( E ) Comparison of viral titers and the total number of viral RNA genomes produced by wild-type and mutant viruses 12 hours after electroporation with in vitro transcribed RNA. The experiment was performed in triplicate, and error bars represent the standard deviation (SD). ( F ) One-step growth curves of wild-type and mCherry-tagged SINV and SINV-ETM chimera at an MOI of 2. Data are representative of two independent experiments.

Article Snippet: Bacterial clones of full-length SINV 6K, SARS-CoV-2 E, EGFP, and E mutant proteins (N15A, V25F, and N15A, V25F double mutant) in pET His6 MBP N10 TEV (Addgene #29706 ) were used for bacterial expression and purification.

Techniques: Mutagenesis, Sequencing, Electroporation, Virus, Software, Infection, Comparison, Produced, In Vitro, Standard Deviation

SINV 6K and SARS-CoV-2 E proteins bind to amiloride derivatives. UV-Vis spectra of ( A ) HMA, EIPA, and DMA. UV-Vis spectra of compounds bound to ( B ) SINV 6K, ( C ) SARS-CoV-2 E, and ( D ) EGFP proteins. Blank refers to the absorbance of the dialysis buffer after unbound compounds have been removed from each sample. The data shown represent two independent experiments.

Journal: Journal of Virology

Article Title: A BSL-2 chimeric system designed to screen SARS-CoV-2 E protein ion channel inhibitors

doi: 10.1128/jvi.02252-24

Figure Lengend Snippet: SINV 6K and SARS-CoV-2 E proteins bind to amiloride derivatives. UV-Vis spectra of ( A ) HMA, EIPA, and DMA. UV-Vis spectra of compounds bound to ( B ) SINV 6K, ( C ) SARS-CoV-2 E, and ( D ) EGFP proteins. Blank refers to the absorbance of the dialysis buffer after unbound compounds have been removed from each sample. The data shown represent two independent experiments.

Article Snippet: Bacterial clones of full-length SINV 6K, SARS-CoV-2 E, EGFP, and E mutant proteins (N15A, V25F, and N15A, V25F double mutant) in pET His6 MBP N10 TEV (Addgene #29706 ) were used for bacterial expression and purification.

Techniques:

Inhibition of SINV and SINV-ETM mutant virus infection by amilorides and amantadine in BHK cells at MOI of 1.0 and 12 h incubation. BHK cells were treated with indicated concentrations of ( A, E ) HMA, ( B, F ) EIPA, ( C, G ) DMA, and ( D, H ) amantadine and infected with ( A–D ) mCherry E2 WT SINV (green), mCherry E2 Δ6K SINV (pink) and ( E–H ) mCherry E2 SINV-ETM (orange), ETM (N15A) (blue), ETM (V25F) (red), ETM (N15A, V25F) (purple) viruses at an MOI of 1.0 for 12 hours. ( I ) BHK-21 cells were infected with untagged WT SINV, ∆6K SINV, and SINV-ETM chimera at an MOI of 1.0 treated with indicated concentrations of inhibitors and plaqued after 12 hours.

Journal: Journal of Virology

Article Title: A BSL-2 chimeric system designed to screen SARS-CoV-2 E protein ion channel inhibitors

doi: 10.1128/jvi.02252-24

Figure Lengend Snippet: Inhibition of SINV and SINV-ETM mutant virus infection by amilorides and amantadine in BHK cells at MOI of 1.0 and 12 h incubation. BHK cells were treated with indicated concentrations of ( A, E ) HMA, ( B, F ) EIPA, ( C, G ) DMA, and ( D, H ) amantadine and infected with ( A–D ) mCherry E2 WT SINV (green), mCherry E2 Δ6K SINV (pink) and ( E–H ) mCherry E2 SINV-ETM (orange), ETM (N15A) (blue), ETM (V25F) (red), ETM (N15A, V25F) (purple) viruses at an MOI of 1.0 for 12 hours. ( I ) BHK-21 cells were infected with untagged WT SINV, ∆6K SINV, and SINV-ETM chimera at an MOI of 1.0 treated with indicated concentrations of inhibitors and plaqued after 12 hours.

Article Snippet: Bacterial clones of full-length SINV 6K, SARS-CoV-2 E, EGFP, and E mutant proteins (N15A, V25F, and N15A, V25F double mutant) in pET His6 MBP N10 TEV (Addgene #29706 ) were used for bacterial expression and purification.

Techniques: Inhibition, Mutagenesis, Virus, Infection, Incubation

Time of inhibitor addition assay at high MOI. ( A ) Schematic representation of the time of addition assay protocol. ( B ) Time of addition assay in BHK-21 cells with channel inhibitors. Cells were infected with either mCherry-E2-tagged SINV or SINV-ETM virus at t = 0 with a high MOI of 10. 25 µM HMA, 25 µM EIPA, 50 µM DMA, and 500 µM Amantadine were added to the cells at the indicated time points. Cells were fixed and imaged at 15 hpi at 4× magnification. The fluorescence intensity signal (TRITC channel) was measured for each sample relative to the DMSO-control signal and plotted for graphical representation. Error bars represent the standard error of the mean (SEM) of two independent experiments. Statistical significance was determined for each time point between inhibitor-treated and DMSO control values by two-tailed unpaired t-test with a 95% confidence interval. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 and ns-not significant.

Journal: Journal of Virology

Article Title: A BSL-2 chimeric system designed to screen SARS-CoV-2 E protein ion channel inhibitors

doi: 10.1128/jvi.02252-24

Figure Lengend Snippet: Time of inhibitor addition assay at high MOI. ( A ) Schematic representation of the time of addition assay protocol. ( B ) Time of addition assay in BHK-21 cells with channel inhibitors. Cells were infected with either mCherry-E2-tagged SINV or SINV-ETM virus at t = 0 with a high MOI of 10. 25 µM HMA, 25 µM EIPA, 50 µM DMA, and 500 µM Amantadine were added to the cells at the indicated time points. Cells were fixed and imaged at 15 hpi at 4× magnification. The fluorescence intensity signal (TRITC channel) was measured for each sample relative to the DMSO-control signal and plotted for graphical representation. Error bars represent the standard error of the mean (SEM) of two independent experiments. Statistical significance was determined for each time point between inhibitor-treated and DMSO control values by two-tailed unpaired t-test with a 95% confidence interval. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 and ns-not significant.

Article Snippet: Bacterial clones of full-length SINV 6K, SARS-CoV-2 E, EGFP, and E mutant proteins (N15A, V25F, and N15A, V25F double mutant) in pET His6 MBP N10 TEV (Addgene #29706 ) were used for bacterial expression and purification.

Techniques: Infection, Virus, Fluorescence, Control, Two Tailed Test